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Logos Biosystems Fluorescein Diacetate/Propidium Iodide Stain (FDA/PI Stain)
FDA/PI Stain is a combination of fluorescent dyes for assessing the viability of diverse cell types, including mammalian cells, yeast, and pollen grains. FDA is a non-fluorescent dye that permeates cell membranes, undergoing hydrolysis by the esterase activity of viable cells and producing the green fluorescent compound fluorescein. FDA can be retained in viable cells with intact membranes, acting as a live cell marker. PI, a red membrane-impermeable dye, selectively penetrates dead or damaged cells with compromised membranes. PI is a commonly used dead cell marker that binds to DNA and RNA through intercalation. Upon binding, the fluorescence intensity of PI can be increased by 20- to 30- fold higher.
Compatible with the following cell counters:
- LUNA-FLâ„¢ Automated Cell Counter
- LUNA-FX7â„¢ Automated Cell Counter
Specifications
| Fluorescein Diacetate Stain | Propidium Iodide Stain | |
| Molecular formula | C24H16O7 | C27H34I2N4 |
| Molecular weight | 416.38 g/mol | 668.39 g/mol |
| Appearance | Clear liquid | Clear liquid |
| Cell permeability | Membrane permeant | Membrane impermeant |
| Excitation/emission | 494/521 nm (when converted to fluorescein) | 493/636 nm (in aqueous solution) 533/617 nm (bound to nucleic acids) |
| Quantity | 1 x 0.5 mL | 1 x 0.5 mL |
| Storage | - 20°C in the dark | 4°C in the dark |