Learn how to use this product biotechrabbit™ CAPITAL qPCR Green Mix enables specific and sensitive amplification with an excellent signal-to-noise ratio and rapid extension rates. Extremely low-copy-number targets can be detected with high efficiency over several logs of template concentration, while the primer-dimer formation is efficiently minimized. CAPITAL qPCR Green Mix performs accurately and robustly in various applications, including gene expression and copy number variation analysis. To enable the use of the kit on qPCR platforms with different reference dye concentration requirements, three formats are available. A kit containing no ROX and versions containing low (LRox) or high (HRox) concentrations of ROX. Info: The recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).